Contact

E-Mail: knief@uni-bonn.de
Fon: +49 228 73 2555
Homepage: https://www.boden.uni-bonn.de/molekularbiologie-der-rhizosphere/mitarbeiter

Research topics

• Microbial ecology of the soil and rhizosphere: Analysis of microbial community composition and abundances, Identification of microorganisms involved in carbon cycling
• Phyllosphere microbiology: Analysis of microbial community composition and abundances, Physiology of phyllosphere-associated microorganisms
• Methylotrophic bacteria: Diversity and physiology of methylotrophic bacteria in their natural habitats

Resources

• Amplicon sequencing: Microbial community analysis based on 16S rRNA genes or functional marker genes
• qPCR: Estimation of bacterial abundances
• DNA-, RNA-, PLFA-SIP: Identification of metabolically active microorganisms by stable isotope probing
• DGGE, ARISA, T-RFLP: Fingerprint methods for the analysis of bacterial community composition
• Cultivation of bacteria

Publications

• Knief, C. (2015): Diversity and habitat preferences of cultivated and uncultivated aerobic methanotrophic bacteria evaluated based on pmoA as molecular marker, Front. Microbiol., 6: 1346
• Knief, C., Delmotte, N., Chaffron, S., Stark, M., Innerebner, G., Wassmann, R., von Mering, C. Vorholt, J. A. (2012): Metaproteomic analysis of microial communities in the phyllosphere and rhizosphere of rice, ISME J., 6: 1378-1390
• Knief, C., Ramette, A., Frances, L., Alonso-Blanco, C., Vorholt, J. A. (2010): Site and plant species are important determinants of the Methylobacterium community composition in the phyllosphere, ISME J., 4: 719-728
• Delmotte, N., Knief, C., Chaffron, S., Innerebner, G., Roschitzki, B., Schlapbach, R., von Mering, C. Vorholt, J. A. (2009): Community proteogenomics reveals insights into the physiology of phyllosphere bacteria, Proc. Natl. Acad. Sci. U S A, 106: 16428-16433
• Knief, C., Lipski, A., and Dunfield P. F. (2003): Diversity and activity of methanotrophic bacteria in different upland soils, Appl. Environ. Microbiol., 69: 6703 – 6714
• full publication list: https://www.boden.uni-bonn.de/molekularbiologie-der-rhizosphere/publikationen